Fig. 4

DUSP3 is associated with ZO-1 and OCLN in a cellular context. A DelA-F13 and F16R cells were subjected to the PLA to examine the close proximity of DUSP3 with OCLN or ZO-1 in the cellular context. B and C PLAs with the presence of only one primary antibody (DUSP3, OCLN, or ZO-1) was performed as the negative control experiments. DAPI was used for nuclear staining. Quantitative numbers were the mean and standard deviations of PLA-positive signals per cells from at least 200 cells