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Fig. 3 | Journal of Biomedical Science

Fig. 3

From: Hepatitis B virus virion secretion is a CRM1-spike-mediated late event

Fig. 3

CRM1 inhibitors blocked secretion of HBV virions but not naked capsids. A Left panel: Secretions of WT-HBV virions and HBsAg particles were strongly inhibited by SINE compound at 0.1 μM in a dose–response curve. While virion secretion was clearly reduced when SINE compound concentration was increased, secretion of naked capsid was far less sensitive to drug treatment. Similarly, Southern blot analysis revealed that the secretion of genome-containing virions was much more sensitive to SINE compounds than the secretion of genome-containing naked capsids. Right panel: Bar graphs were plotted based on the signal intensities of HBc, HBs and HBV DNA, as measured by a densitometer and the Image J Software. The values were calculated as mean of more than three independent experiments. B Left panel: As a control to the extracellular secretion profiles in (A), we examined the total intracellular lysate for the WT-HBV capsid protein, capsid-associated RNA and DNA, in the same dose–response experiment by the SINE compound treatment. Right panel: HBV specific signals were plotted as a bar graph. The values were calculated as mean of more than three independent experiments

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