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Fig. 6 | Journal of Biomedical Science

Fig. 6

From: Hepatitis B virus virion secretion is a CRM1-spike-mediated late event

Fig. 6

HBV virion secretion is microtubule dependent. A HBV virions secretion can be inhibited by Nocodazole treatment at low concentration (1 μM). In contrast, secretion of naked capsids was highly resistant to the Nocodazole treatment. The bar graph (right panel) is based on the signal intensities of virions and naked capsids (left panel) using densitometry and Image J. The mean values were calculated from three independent repeat experiments. HBV virion secretion, but not naked capsid secretion, could be microtubule-dependent. B Potential CRM1 and microtubule interactions were measured by detecting the immunofluorescence using the Duolink® PLA reagents (left panel). The bar graph (right panel) indicates the CRM1/Microtubule PLA interactions (< 40 nm) per cell by MetaMorph Microscopy Automation and Image Analysis Software. Respective cell numbers scored: no treatment (337 cells), SINE (385 cells). SINE compound treatment decreased the CRM1 and microtubule interactions. Student t test was used for statistics. C HBc and CRM1 interactions were measured similarly by Duolink® PLA reagents. HBc and CRM1 accumulated in the nucleus after SINE compound treatment. Cell numbers scored: no treatment (849 cells), SINE (325 cells). D HBc and microtubule interactions were measured by Duolink® PLA reagents. SINE compound treatment decreased the HBc and microtubule interactions. Cell numbers scored: no treatment (565 cells), SINE (413 cells)

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