Fig. 7
Cell cycle-regulated protein Trap1 is downregulated in the loss of XBP1 models. a Protein expression levels in XBP1 deficient HK-2 were presented by MS-based proteomics analysis. The present data shows siXBP1 over siNTC protein expression normalized ratio. b and c The protein expression of Trap1 in XBP1 silenced HK-2 cell was examined by western blot and quantified. GAPDH was used as an internal control. Data are expressed as means ± SEM of three independent experiments. *P < 0.05. d and e The protein expression of Trap1 in mice kidneys of UIRI was evaluated with western blotting and quantified. f and g The protein expression of Trap1 in mice kidneys of UUO was evaluated with western blotting and quantified. h and i The protein expression of Trap1 in mice kidneys of adenine diet was evaluated with western blotting and quantified. j and k The protein expression of Trap1 in XBP1cKO mice kidneys was evaluated with western blotting and quantified. Data are expressed as means ± SEM of at least three independent measurements. *P < 0.05, and ***P < 0.001, as compared with sham group