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Fig. 9 | Journal of Biomedical Science

Fig. 9

From: HrpA anchors meningococci to the dynein motor and affects the balance between apoptosis and pyroptosis

Fig. 9

Effects of infection of HeLa cells by N. meningitidis B1940 or B1940ΩhrpA strains on apoptosis and pyroptosis markers. A Lysates from HeLa cells transfected with control RNA and with two different DYNLT1 siRNAs (indicated as #1 and #2) were subjected to SDS-PAGE and analyzed by immunoblotting using specific antibodies for Caspase-8, Caspase-3, N-terminal Gasdermin E (GSDME), Caspase-4, Caspase-1, and Gasdermin D (GSDMD). Antibodies against GAPDH were used to verify loading while antibodies against DYNLT1 were used to check dynein silencing. B, D, F, H, J, L Signal intensity was quantified by densitometric analysis and reported as fold changes of DYNLT1 siRNA samples compared to their controls. Values are the mean ± SEM of at least three independent experiments. *P < 0.05 **P < 0.01 ***P < 0.001. C, E, G, I, K, M Signal intensity was quantified by densitometric analysis and reported as fold changes of infected samples compared to non-infected cells or compared to wild type if no signal was detected in non-infected cells. Values are the mean ± SEM of at least three independent experiments. *P < 0.05 **P < 0.01 ***P < 0.001

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