Fig. 2

A Fold-changes relative to control group of vegfr2 mRNA expression in cultured mouse neurons detected at different time-points (24 or 48 h) after treatment with different concentrations (10 or 20 nM) of vegfr2 specific siRNA (si-VEGFR2) or control nonspecific siRNA (si-Control; 20 nM). Values are mean ± SEM of 3 independent experiments. *p < 0.05 versus control (non-treatment) group in the Dunnett multiple-range test; ⁺p < 0.05 versus si-Control group in the Tukey’s multiple-range test. B Schematic diagram of siRNA treatment experiments. DIV, days in vitro. C Effects of treatment with si-VEGFR2 or si-Control on fold-changes relative to control (non-NH₄Cl treatment) group of vegfr2 mRNA expression in cultured neurons incubated with ammonia (5 mM NH₄Cl). Values are mean ± SEM of 3–4 independent experiments. *p < 0.05 versus non-treatment group in the Dunnett multiple-range test; ⁺p < 0.05 versus si-Control group in the Tukey’s multiple-range test. D Effects of treatment with si-VEGFR2 or si-Control on cell proliferation measured by the WST-1 assay in cultured neurons incubated with or without ammonia. Values are mean ± SEM of 3–4 independent experiments. *p < 0.05 versus non-treatment group in the Dunnett multiple-range test; ⁺p < 0.05 versus non-NH₄Cl treatment group at corresponding group in the Tukey’s multiple-range test; ^#p < 0.05 versus si-Control treatment group in the Tukey’s multiple-range test