Fig. 3
Synaptic gephyrin levels and gephyrin-GABAAR associations in two generations of the VPA-induced offspring. a, b Representative western blot profile and the protein levels of gephyrin (a) and GABAAR \({\upbeta }\)3 subunit (b) in synaptoneurosomes from the amygdala. Gephyrin, n = 8 rats from three or four litters for each condition; GABAAR \({\upbeta }\)3 subunit, n = 7 rats from three or four litters for each condition. c, d Representative western blot profile and the protein levels of gephyrin (c) and GABAAR \({\upbeta }\)3 subunit (d) in whole-cell lysate from the amygdala. Gephyrin, n = 6 rats from three or four litters for each condition; GABAAR \({\upbeta }\)3 subunit, n = 6 rats from three or four litters for each condition. (e) Representative western blot profile and the protein levels of co-immunoprecipitation of gephyrin and GABAAR \({\upbeta }\)3 subunit in synaptoneurosomes from the amygdala. n = 6 rats from three or four litters for each condition. Data are presented as mean ± SEM; *p < 0.05, **p < 0.01, ***p < 0.001 vs. indicated control; one-way ANOVA with Bonferroni post-hoc