Fig. 4
NMDA-induced gephyrin and GABAAR enhancements in two generations of the VPA-induced offspring. a–d Representative western blot profile, the protein levels (a, c), and the main effect plots of two-way ANOVA (b, d) of gephyrin and GABAAR \({\upbeta }\)3 subunit in synaptoneurosomes from the amygdala. Gephyrin, n = 7 rats from three or four litters for each condition; GABAAR \({\upbeta }\)3 subunit: n = 9 rats from three or four litters for each condition. e Representative mIPSCs traces from each group. Scale bars, 100 pA, 50 ms. f, i Bar graphs show average mIPSC amplitudes (f) and frequency (i) measured from the BLA pyramidal neurons. g, j Cumulative probability of mIPSC amplitudes (g) and frequency (j) measured from the BLA pyramidal neurons. h, k The main effect plots of two-way ANOVA of mIPSC amplitudes (h) and frequency (k) measured from the BLA pyramidal neurons. n = 8–10 cells from 4–5 rats from three or four litters for each condition. Data are presented as mean ± SEM; *p < 0.05, ***p < 0.001 vs. indicated control; one-way ANOVA or two-way rmANOVA with Bonferroni post-hoc