Fig. 1

Hesperetin extends lifespan and enhances Cisd2 levels in old WT mice. A The survival rate of WT mice without any treatment (n = 20 mice) and old WT mice treated with vehicle (Veh; n = 8) or hesperetin (Hes; n = 19). For the hesperetin treatment, 21-month old WT mice were treated with dietary hesperetin (100 mg/kg/day) or Veh control food. Statistical comparison of the survival curves by log-rank test: Veh versus hesperetin, p = 0.04; no treatment WT versus hesperetin, p = 0.029; no treatment WT versus Veh, p = 0.5 (n.s.). B–D Elevation of Cisd2 protein levels in multiple tissues of old mice fed with dietary hesperetin at old age. Western blot of Cisd2 in the femoris (B), gastrocnemius (C), and cardiac muscles (D) of 26-month mice treated with dietary hesperetin or Veh control food for 5 months (from 21-month old). The protein level of Cisd2 in 3-month WT mice serves as a young mouse control. Data are presented as mean ± SD. *p < 0.05; **p < 0.005 by one-way ANOVA with Bonferroni multiple comparison test. All the mice used in this study are males