Fig. 4
From: TRIM18 is a critical regulator of viral myocarditis and organ inflammation
Knockout of TRIM18 protects mice from pneumonia and lung injury induced by RNA virus Flu PR8 or DNA virus adenovirus in vivo. a Hematoxylin and eosin (H&E)-staining of lung sections from age- and sex-matched Trim18+/+ (WT) and Trim18−/− (KO) mice left infected (Mock) or infected for 4 days by intranasal infection of Flu PR8 virus (1 × 105 PFU per mouse). b Histology score analysis of viral pneumonia in lung sections from mice as in (a). c Survival of age- and sex-matched WT and KO mice after intranasal infection with Flu PR8 virus (1 × 105 PFU per mouse) (n = 10 per group). d Plaque assay of Flu PR8 virus titers in the lung of WT and KO mice infected for 2 days by intranasal infection of Flu PR8 virus (n = 5 per group). e–f, ELISA of and IFN-α e and IFN-β f in BALF samples from mice (n = 5 per group) as in (d). g Quantification of cell numbers in BALF samples from mice (n = 3 per group) as in (d). h Hematoxylin and eosin (H&E)-staining of lung sections from WT and KO mice left infected (Mock) or infected for 4 days by intranasal infection of adenovirus (1 × 108 PFU per mouse). i, Histology score analysis of viral pneumonia in lung sections from mice as in (h). j Viral titers in homogenates of lung from WT and KO mice (n = 5 per group) at day 2 of intranasal infection with adenovirus (1 × 108 PFU per mouse). k, l ELISA of IFN-α k and IFN-β l in BALF samples from mice as in j (n = 5 per group). m Quantification of cell numbers in BALF samples from mice (n = 3 per group) as in (j). Scale bars represent 400 μm for images in a and (h). Error bars indicate standard error of the mean for results in (b, d–g, i–m). NS, not significant (p > 0.05), **P < 0.01 and ***P < 0.001, and p value was calculated by unpaired two-tailed Student’s t test and Gehan-Breslow-Wilcoxon test for survival analysis. Data are representative of three independent experiments