Fig. 6
From: Homozygous mutation of the LRRK2 ROC domain as a novel genetic model of parkinsonism
LRRK2 R1441G HOM mice accumulated more autophagosomes in the SNc. A Transmission electron microscopic images of autophagosomes from the SN of 12-month-old hLRRK2 and R1441G transgenic mice. Selected regions in images at different magnifications (I, 10,000×; II, 20,000×). Arrows indicate autolysosomes, and asterisks indicate autophagosomes. Markers of autophagy (LC3 and p62) in the SNc of hLRRK2 and R1441G transgenic mice were determined by western blotting. p62 undergoes degradation at the early phase of autophagy. p62 in mitochondria serves as an adapter for autophagosome recognition. The data appear to downregulate the autophagy process, as observed by the increasing LC3-II conversion and the accumulation of p62, a marker of autophagic degradation