Fig. 5

Immune response of the DDS-specific TCR transfectants to DDS and HLA-B*13:01. A–F ELISA was used to quantify the amount of IFN-γ, IL-3, IL-13, IL-5, IL-21 and granzyme B in DDS and DMSO stimulated CTL. G, H the expression levels of TRAV12-3 and TRBV28 were determined by qPCR assay, respectively. 10 patients with DDS DIHS, 10 dapsone tolerant control, and 10 health donors were enrolled. Fold change were calculated via 2^−ΔΔct method after normalization to GAPDH. I IFN-γ ELISPOT response was observed in the co-cultures of PBMC with TCR transfectants, C1R-B*13:01 or EVB-HLA-B*13:01 cells. These results suggested that the special αβTCR reacts to DDS and its structural analogs, and the HLA-B*13:01 presenting promotes the immune recognition. The entire experiment was repeated thrice. *P < 0.05, **P < 0.01, ***P < 0.001, by two-tailed Student t test