Fig. 2

Crista remodeling by CYP11A1 is a conserved function among vertebrates. A Immunohistochemistry images of zebrafish Cyp11a1-EGFP and Cyp11a2-EGFP transfected in COS1 cells showing localization of EGFP in mitochondria. TOM20 (red) is a marker for mitochondria, and DAPI (blue) stains the nucleus. B TEM images of mitochondria of COS1 cells transfected with human CYP11A1 and zebrafish Cyp11a1 and Cyp11a2 fused to EGFP-APEX. C Quantification of tubulovesicular structure observed under TEM. More than 50 cells were counted for quantification of each sample. Unpaired t-test was used, and data shown are mean with standard deviation