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Fig. 4 | Journal of Biomedical Science

Fig. 4

From: Down-regulated miR-146a expression with increased neutrophil extracellular traps and apoptosis formation in autoimmune-mediated diffuse alveolar hemorrhage

Fig. 4

Reduced NETosis in miR-146a-overexpressed dHL-60 cells. a dHL-60 cells stimulated with PMA and stained with Sytox Green to detect DNAs morphology. Upper, Representative photographs from mock and PMA stimulation. Scale bar = 60 µm, magnification × 200. Lower, quantification of NETosis percentages with the diffused/spread NETs morphology. b MiR-146a (left), SNHG16 (middle left), CitH3 (middle right) and PAD4 (right) levels in dHL-60 cells stimulated with PMA for different times. c MiR-146a levels in LV-miR-146a-transfected HL-60 cells. d Quantification of NETosis percentages (left), CitH3 (middle) and PAD4 (right) levels in miR-146a-overexpressed dHL-60 cells under PMA stimulation. The presence of Cl-amidine as a PC. Values are mean ± SEM. All results in this figure were representative of 3 independent experiments with similar findings. * p < 0.05, ** p < 0.01, *** p < 0.001

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