Fig. 7

Down-regulated miR-146a expression with increased NETs formation in mouse neutrophils. a MiR-146a levels in thioglycolate-induced neutrophils purified from saline- and pristane-injected mice on day 0, 4, 9 and 14. b, MIR-146a levels in neutrophils stimulated with variable concentrations of βCD-pristane or 1 μg/mL LPS. c Left upper, representative photographs from neutrophil under 133 μM βCD-pristane or 1 μg/mL LPS stimulation. Scale bar = 30 µm, magnification × 400. Left lower, quantification of NETs morphology. Right, CitH3 levels in supernatants. d MiR-146a expression in neutrophils stimulated with various concentrations of IL-6 or 1 μg/mL LPS. e MIR-146a (left), TRAF6 (middle) and IL-8 (right) levels in neutrophils stimulated with variable concentrations of HMGB1 or 5 μg/mL LPS. f Left upper, representative photographs from neutrophil under 900 ng/mL HMGB1 or 5 μg/mL LPS stimulation. Scale bar = 30 µm, magnification × 400. Left lower, quantification of NETs morphology. Right, CitH3 levels in supernatants. Values are mean ± SEM. 5 mice per group in a. All results in Fig. 7 were representative of 2 independent experiments with similar findings. * p < 0.05, ** p < 0.01, *** p < 0.001