Fig. 8

Reduced apoptosis and HMGB1 production in miR-146a-overexpressed alveolar cells. a Left, representative photographs of TUNEL IF staining (green) in MLE-12 cells stimulated with 400 μM βCD-pristane or 1 μM Dox. Cell nuclei counterstained with DAPI (blue). Scale bar = 30 µm, magnification × 600. Middle, quantification of TUNEL-positive cell percentages. Right, HMGB1 supernatant levels from stimulated MLE-12 cells. b Apoptotic cell ratios (left), HMGB1 supernatant levels (middle left), miR-146a (middle right) and SNHG16 (right) expression in MLE-12 cells stimulated with various concentrations of Dox. c MiR-146a expression in MLE-12 cells stimulated with various concentrations of IL-6. d Left, MiR-146a expression in LV-miR-146a-transfected MLE-12 cells. Middle left, representative photographs of annexin V IF staining (pink) in miR-scr- or miR-146a-overexpressed MLE-12 cells under 1 μM Dox stimulation. Cell nuclei counterstained with Hoechst 33258 (blue). Scale bar = 10 µm, magnification × 1000. Middle right, quantification of apoptotic cell percentages. Right, HMGB1 supernatant levels from miR-scr- or miR-146a-overexpressed MLE-12 cells under 1 μM Dox stimulation. Values are mean ± SEM. All results in this figure were representative of 3 independent experiments with similar findings. * p < 0.05, ** p < 0.01, *** p < 0.001