Fig. 9

DAH suppressed by intra-pulmonary miR-146a delivery through reducing NETs and apoptosis formation. a Left, representative gross and histopathological photographs in the lungs with no, partial and complete hemorrhage. Right, hemorrhagic frequencies of LV-miR-scr- and LV-miR-146a-treated mice. Scale bar = 100 µm, magnification, × 100. b Neutrophils, RBC numbers, Hct and Hb levels in LV-miR-scr- and LV-miR-146a-treated mice. c Left, representative TUNNEL IF staining (green) in lung tissues from LV-miR-scr- and LV-miR-146a-treated mice. Scale bar = 20 µm, magnification, × 400. Right, quantification of TUNEL-positive cell numbers in lung tissues. d MiR-146a levels in lung and spleen tissues from LV-miR-scr- and LV-miR-146a-treated mice. e Levels of IL-6, IL-8, IFN-α and MX-1 in lung tissues from LV-miR-scr- and LV-miR-146a-treated mice. f Representative immunoblot assay (right) with signal intensity quantitation (left) of pulmonary CitH3, PAD4, TRAF6, IRAK1 and β-actin levels from saline- and pristane-injected mice. Values are mean ± SEM. Horizontal lines are mean values. Mouse numbers per group, 16 in a, b, 5 in c, 8 in d, 8 in e, 4 in f. All results in this figure were representative of 2 independent experiments with similar findings. * p < 0.05, ** p < 0.01, *** p < 0.001