Fig. 6

LGp40 failed to inhibit M2 macrophages in HDM-induced asthma when the enzymatic activity was lost. Lung sections were immunostained with a iNOS and b arginase-1 antibodies then reacted with diaminobenzidine (Scale bars = 200 and 100 μm). The iNOS-positive cells (blue arrows) were counted in every lobe per group, and the average numbers were calculated. The arginase-1-positive cells (red arrows) were counted in ×200 microscopic fields per group. The average numbers were calculated (n = 5 mice, **p < 0.01, one-way ANOVA with Bonferroni multiple comparison test). c IL-6, d IL-12p40, and e IL-10 production in cultured supernatants of IL-4- and IL-13-stimulated BMDM incubated with recombinant proteins were determined using an ELISA assay kit. f IL-4- and IL-13-stimulated BMDM were incubated with naïve Th cells in the presence of recombinant proteins. IL-4⁺ Th2 cell populations were determined using flow cytometry analysis (n = 6 mice, *p < 0.05 and **p < 0.01, one-way ANOVA with Bonferroni multiple comparison test). Results comprise pooled data from three independent experiments