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Fig. 7 | Journal of Biomedical Science

Fig. 7

From: Moonlighting glyceraldehyde-3-phosphate dehydrogenase (GAPDH) protein of Lactobacillus gasseri attenuates allergic asthma via immunometabolic change in macrophages

Fig. 7

LGp40, rather than CDp40, triggered glycolysis in allergic M2 macrophages. a Lactate production in cultured supernatants of IL-4- and IL-13-stimulated BMDM that were incubated with recombinant proteins were determined using an ELISA assay kit (n = 6 mice, *p < 0.05 and **p < 0.01, one-way ANOVA with Bonferroni multiple comparison test). Results are pooled data from three independent experiments. b The superimposed structures of LGp40 (green) and CDp40 (cyan) in a schematic representation. The key residues surrounding the NAD-binding sites of LGp40 and CDp40 are indicated. The side chains of the residues around the NAD(P)-binding sites and NAD+ molecule are shown as ball-and-stick models. The carbon atoms of the LGp40, the CDp40, and NAD+ molecules are shown in yellow, light gray, and magenta, respectively. Oxygen atoms are shown in red, nitrogen in blue, and phosphate in orange. The residues marked at the front (A36, T123, C155, H182, T185, R236, and N318) are key residues that surround the NAD(P)-binding site of CDp40. The corresponding residues (D38, S125, C156, H183, T186, R239 and N320) in LGp40 are marked in back

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