Fig. 6

Characterization of apoptosis of HPV18 E6 peptide (aa97-105), or HPV 18 E7 peptide pulsed TC-1/AAD cells induced by HPV18 E6 peptide (aa97-105), or HPV 18 E7 peptide (aa7-15)-specific CD8 + T cells. To detect the apoptosis induced by HLA-A2-restricted HPV18 E6 peptide (aa97-105), or HPV 18 E7 peptide (aa7-15)-specific CD8 + T cells, TC-1/AAD cells were pulsed with either HPV18 E6aa97-105, or HPV 18 E7aa7-15 peptide. After wash, these cells were co-cultured with either HPV18 E6 peptide (aa97-105), or HPV 18 E7 peptide (aa7-15)-specific CD8 + T cells at indicated E:T ratio for 4 h at 37 °C. The cells were then stained with FITC-conjugated anti-mouse CD45 and PE-conjugated Annexin V in annexin binding buffer followed by flow cytometry analysis. Data were analyzed on CD45 negative cells. A Flow cytometry analysis of apoptosis of HPV18 E7 peptide (aa7-15) or HPV18 E6 peptide (aa97-105) pulsed TC-1/AAD cells by HPV18 E7 peptide (aa7-15)-specific CD8 + T cells. B Summary of flow cytometry analysis of apoptosis of TC-1/AAD cells by HPV18 E7 peptide (aa7-15)-specific CD8 + T cells. C Flow cytometry analysis of apoptosis of HPV18 E7 peptide (aa7-15) or HPV18 E6 peptide (aa97-105) pulsed TC-1/AAD cells by HPV18 E6 peptide (aa97-105)-specific CD8 + T cells. D Summary of flow cytometry analysis of apoptosis of TC-1/AAD cells by HPV18 E6 peptide (aa97-105)-specific CD8 + T cells