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Fig. 8 | Journal of Biomedical Science

Fig. 8

From: Delivering genes with human immunodeficiency virus-derived vehicles: still state-of-the-art after 25 years

Fig. 8

Schematic overview of CRISPR/Cas9 delivery using lentiviral vectors. A Cas9 and sgRNA are delivered within a single lentiviral transfer vector (lentiCRISPR-v2). Integration of the lentiCRISPR-v2 vector in the genome of target cells results in persisting expression of both Cas9 and sgRNA, which can then act on the genome of target cells. B Using integrase-defective lentiviral vectors (IDLVs), the lentiCRISPR-v2 vector remains episomal upon transduction, which results in only transient expression of both Cas9 and sgRNA, thus minimizing potential unwanted off-target effects. C In addition to delivering Cas9 and sgRNA, IDLVs can also be utilized to deliver a repair template (donor) for homology-directed repair (HDR)

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