Fig. 8From: Delivering genes with human immunodeficiency virus-derived vehicles: still state-of-the-art after 25 yearsSchematic overview of CRISPR/Cas9 delivery using lentiviral vectors. A Cas9 and sgRNA are delivered within a single lentiviral transfer vector (lentiCRISPR-v2). Integration of the lentiCRISPR-v2 vector in the genome of target cells results in persisting expression of both Cas9 and sgRNA, which can then act on the genome of target cells. B Using integrase-defective lentiviral vectors (IDLVs), the lentiCRISPR-v2 vector remains episomal upon transduction, which results in only transient expression of both Cas9 and sgRNA, thus minimizing potential unwanted off-target effects. C In addition to delivering Cas9 and sgRNA, IDLVs can also be utilized to deliver a repair template (donor) for homology-directed repair (HDR)Back to article page