Fig. 4

The effects of SOX17 and/or NRF2 overexpression on cell behaviors and tumor growth. A, B Colony formation assay of KYSE510 pair cells manipulated with SOX17 and/or NRF2 expression. The colonies were stained on day 8 after seeding (A), and the colony formation ability was quantified (B). C, D Wound healing assay of KYSE510 pair cells manipulated with SOX17 and/or NRF2 expression. Cells were monitored for their ability to migrate into the wound gap. The wound gap was photographed (C) and quantified (D) at 10 h. E, F Transwell invasion assay of KYSE510 pair cells manipulated with SOX17 and/or NRF2 expression. The invaded cells were photographed (E) and quantified (F) at 20 h. G KYSE510-R cells transfected with SOX17 and/or NRF2 were subcutaneously injected into BALB/c nude mice and observed for tumor growth. H, I Tumor size (H) and tumor weight (I) were measured at the end of the experiment. Data represents mean ± s.e.m. ns: non-significant; *p < 0.05; **p < 0.01; ***p < 0.001