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Fig. 2 | Journal of Biomedical Science

Fig. 2

From: Hepatitis B virus suppresses complement C9 synthesis by limiting the availability of transcription factor USF-1 and inhibits formation of membrane attack complex: implications in disease pathogenesis

Fig. 2

HBX downregulates C9 promoter activity and USF-1 expression. a Relative expression of C9 in HBx transfected Huh7 cells with or without DNA methylation inhibitor 5-aza-2’-deoxycytidine (5 μM) or histone deacetylation inhibitor Trichostatin A (0.1 μM). b Relative luciferase activity of C9 promoter-luciferase reporter construct (pGL3-C9-Prom) when co-transfected with HBx-expressing plasmid [pcDNA3.1/myc-His(B)-HBx] or empty vector. c CpG island prediction in C9 promoter using Methprimer online software. Relative expression of d USF1and ATF2 mRNA and e USF-1 protein in HBx- or empty vector transfected Huh7 cells measured respectively by real time PCR and western blot analysis using anti-USF1 antibody. Mean ± SD are presented based on three independent experiments. Paired t tests were performed for comparing paired groups. *p < 0.05, ***p < 0.0001

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Journal of Biomedical Science

ISSN: 1423-0127