Skip to main content
Fig. 5 | Journal of Biomedical Science

Fig. 5

From: Hepatitis B virus suppresses complement C9 synthesis by limiting the availability of transcription factor USF-1 and inhibits formation of membrane attack complex: implications in disease pathogenesis

Fig. 5

Reduced MAC formation and Huh7 cell lysis with CHB sera were reversed upon C9 supplementation. a Representative immunofluorescence images at ×40 magnification showing MAC formation (C5b-9, green) on HBV transfected Huh7 cells treated with serum from HC and CHB patient as well as CHB serum supplemented with C9 protein. The nucleus was counterstained with DAPI (blue). b Viability of HBV transfected Huh7 cells measured by MTT assay upon incubation with HC sera, CHB sera and C9 supplemented CHB sera. The absorbance readings were averages of triplicate experiments. c Correlation analysis between serum C9 concentration and HBV-DNA levels in chronically HBV infected individual. One-way ANOVA followed by Tukey’s multiple comparison were used in (b). **p < 0.005, ***p < 0.0001

Back to article page