From: Spatial multi-omics analyses of the tumor immune microenvironment
Spatial transcriptomic technique | Biomolecule target | Read-out | Resolution | Coverage | Number of targets | Tissue preparation | References |
---|---|---|---|---|---|---|---|
NGS based | |||||||
 10X Visium | RNA | Sequencing | 55 μm | Full |  > 10,000 | FFPE, FF | [32] |
 Slide-seq | RNA | Sequencing | 10 μm | Full |  > 10,000 | FF | [33] |
 Slide-seq V2 | RNA | Sequencing | 10 μm | Full |  > 10,000 | FF | [34] |
 XYZseq | RNA | Sequencing | Single cell in 500 μm microwells | Full | 10,000 | FFPE, FF | [35] |
 HDST | RNA | Sequencing | 2 μm | Full |  > 10,000 | FF | [36] |
 Stereo-seq | RNA | Sequencing | 0.22 μm on chip | Full |  > 10,000 | FF | [38] |
 ZipSeq | RNA | Sequencing | Single cell | Full |  > 10,000 | live cells | [40] |
 Pick-seq | RNA | Sequencing | 5–20 cells | Full |  > 10,000 | FFPE, FF | [44] |
 DBiT-seq | RNA | Sequencing | 10 μm | Full |  > 10,000 | FF | [41] |
 Seq-Scope | RNA | Sequencing |  ~ 0.6 μm on chip | Full |  > 10,000 | FF | [37] |
In situ sequencing based | |||||||
 FISSEQ | RNA | Sequencing by ligation | Sub-cellular | Full | 16,000 | FFPE, FF | [46] |
 STARmap | RNA | Sequencing by ligation | Single cell | Targeted | 1020 | FF | [47] |
In situ hybridization based | |||||||
 MERFISH | RNA | Cyclic imaging | Sub-cellular | Targeted | > 10,000 | FF | [48] |
 Seq-FISH +  | RNA | Cyclic imaging | Sub-cellular | Targeted | > 10,000 | FF | [52] |
 DSP | RNA | Sequencing | 200 μm | Targeted | 1600 | FFPE | [43] |