Spatial multi-omics analyses of the tumor immune microenvironment

Table 2 Spatial proteomics platforms

Spatial proteomic technique	Biomolecule target	Read-out	Resolution	Number of targets	Tissue preparation	References
Fluorophore-based
MICS	Protein	Cyclic imaging	Sub-cellular	100	FFPE	[53]
t-CycIF	Protein	Cyclic imaging	Single cell	60	FFPE	[54, 55]
CODEX	Protein	Cyclic imaging	Single cell	60	FFPE, FF	[56]
Immuno-SABER	Protein	Cyclic imaging	Sub-cellular	10	FFPE, FF	[57]
InSituPlex	Protein	Cyclic imaging	Sub-cellular	5	FFPE	[58]
MOSAICA	Protein/ Nucleic acid	Spectral and time resolved fluorescence imaging	Single cell	Up to 10	FFPE	[59]
Metal-based
IMC	Protein	Mass cytometry	1 μm	40	FFPE, FF	[60]
MIBI	Protein	Mass cytometry	260 nm	40	FFPE, FF	[61]

ISSN: 1423-0127