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Fig. 1 | Journal of Biomedical Science

Fig. 1

From: Interference in melanoma CD248 function reduces vascular mimicry and metastasis

Fig. 1

Melanoma CD248 promotes cell adhesion and migration. Mouse melanoma cells (B16F10 cells) transfected with siRNA against mouse Cd248 (siCd248) or with scrambled control siRNA (siCtrl) were subjected to cell adhesion and migration assays. A Western blot analysis of CD248 expression in B16F10 cells treated with the fibroblast-conditioned medium (CM) for 1 day and the statistical analysis thereof. N = 5. ***P < 0.001. B Western blot analysis of CD248 expression in B16F10 cells transfected with siCtrl or siCd248. N = 5. ***P < 0.001. The cells were subjected to C, D cell adhesion assay, E chemotactic migration assay, and F wound recovery assay. C Western blot analysis of FAK activation (phosphorylation of Tyr576 FAK, p-FAK(Tyr576) in cells after cell adhesion assay. N = 5. ***P < 0.001. D Representative images of cells 30 min after adhesion assay to fibronectin (FN)-coated multiple-well plate and the statistical analysis thereof. The level of cell adhesion was determined by measuring the number of adherent cells after staining with crystal violet followed by the measurement of OD570nm. N = 5. ***P < 0.001. E Representative images of migrated cells 3 h after chemotactic migration followed by Liu’s stain. Chemotaxis was induced by the CM. Statistical analysis of chemotaxis assay. N = 5. ***, P < 0.001. F Representative images of wound recovery assay and statistical analysis thereof. N = 8. *P < 0.05; ***P < 0.001

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