Fig. 2
From: Interference in melanoma CD248 function reduces vascular mimicry and metastasis
Melanoma CD248 promotes vascular mimicry but does not affect cell growth. A Analysis of the correlation of mRNA expression between CD248 and molecular markers for VM in the human melanoma tissues using the cBioPortal database. B16F10 cells transfected with siCtrl or siCd248 were subjected to B western blot assay, C, D VM assay (network formation on Matrigel), and E cell proliferation assay (MTT assay). B Western blot analysis of the relative expression of CD248 and MMP9 in cells treated with fibroblast-cultured conditioned medium (CM) with non-cell cultured medium (SF) as the control for 1 day. N = 6. *P < 0.05; **P < 0.01; ***P < 0.001. C Representative images of VM assay induced by CM with SF as control. D Statistical analysis of the total length of the network measured 6 h after seeding on Matrigel. N = 5. ***P < 0.001. E MTT assay. B16F10 cells with siCd248 or siCtrl were cultured for 1 to 3 days and followed by an MTT assay at indicated time point. N = 4