Fig. 5

Nontoxicity of oleuropein in mice. a No growth retardation of mice was evident with oleuropein (25 mg/kg, once a day) treatment. The body weight of female mice was determined during vehicle versus oleuropein treatment. b–j, Liver panel assay of the blood of female mice treated with oleuropein (25 mg/kg, once a day for 21 days) versus the vehicle. Levels of albumin (ALB, b), total protein (TP, b), total bilirubin (TBIL, c), direct bilirubin (DBIL, c), aspartate aminotransferase (AST, d), lactate dehydrogenase (LDH, e), alkaline phosphatase (ALP, f), alanine aminotransferase (ATL, g), globulin (GLOB, h), albumin-globulin ratio (A/G, i), and indirect bilirubin (IBIL, j) in the blood of C57BL/6J female mice treated with the vehicle (Veh) or oleuropein (OLE). k Litter size of C57BL/6J female mice treated with the vehicle or oleuropein (25 mg/kg). *, p < 0.05; NS nonspecific