Fig. 2

Deficient decidualization in mice and human with sleep disturbance. a The representative picture of uterus from mice with normal or sleep disturbance after artificial decidualization. b HE staining for cross section of uterus from mice with normal or sleep disturbance after artificial decidualization. c The relative mRNA level of decidualization markers (Dtprp, Wnt4, Bmp2) in oil-injected lateral uterus of mice with normal or sleep disturbance. d, e The relative mRNA level of Rev-erbα and IGFBP1 in DSCs of human normal pregnancies with normal sleep and miscarriages with sleep disturbance. f The protein level of Rev-erbα and IGFBP1 in DSCs of human normal pregnancies with normal sleep and miscarriages with sleep disturbance. Relative protein levels were normalized to β-Tubulin. g The protein level of Rev-erbα and IGFBP1 in hESCs of human with normal or sleep disturbance after in vitro decidualization. Relative protein levels were normalized to β-Actin. mNS represented mouse with normal sleep. mSD represented mouse with sleep disturbance. hNS represented human with normal sleep. hSD represented human with sleep disturbance. hNP represented human with normal pregnancy and normal sleep. hMis represented human with miscarriage and sleep disturbance. Data represented Mean ± SEM. Statistical analysis was performed using Student’s t‐test. *P < 0.05, **P < 0.01, ***P < 0.001