Fig. 4

Rev-erbα knockdown downregulated PR and C/EBPβ expression in hESCs. a KEGG analysis results of differentially expressed genes between hESCs without Rev-erbα knockdown and that with Rev-erbα knockdown. b The relative mRNA level of PGR and C/EBPβ in hESCs with or without Rev-erbα knockdown. c and d The protein level of PR and C/EBPβ in hESCs with or without Rev-erbα knockdown. Relative protein levels were normalized to β-Actin (in c) or β-Tubulin (in d). e The relative mRNA level of PGR, C/EBPβ and IGFBP1 in hESCs with or without PGR knockdown. f The protein level of C/EBPβ in hESCs with or without PGR knockdown. Relative protein levels were normalized to β-Actin. g The protein level of IGFBP1 in hESCs with or without C/EBPβ knockdown. Relative protein levels were normalized to β-Actin. Data represented Mean ± SEM. Statistical analysis was performed using Student’s t‐test. *P < 0.05, **P < 0.01, ***P < 0.001