Fig. 5

Rev-erbα regulated decidualization via IL-6-PR-C/EBPβ axis in hESCs. a The protein level of IL-6 and IL-6R in hESCs with or without Rev-erbα knockdown. Relative protein levels were normalized to β-Tubulin. b Chip-PCR assay showing recruitment of Rev-erbα to IL-6 and IL-6R promoter in hESCs. c, d The relative mRNA level of PGR and C/EBPβ in hESCs stimulated with different concentrations of IL-6. e The protein level of PR, C/EBPβ and IGFBP1 in hESCs with IL-6 stimulation. Relative protein levels were normalized to β-Actin. f IL-6 neutralized antibody (anti-IL-6) reversed the decreased PR, C/EBPβ and IGFBP1 expression in hESCs with Rev-erbα knockdown. Relative protein levels were normalized to β-Actin. g IL-6R inhibitor alleviated the decreased PR, C/EBPβ and IGFBP1 expression in hESCs with Rev-erbα knockdown. Relative protein levels were normalized to β-Actin. Data represented Mean ± SEM. Statistical analysis was performed using Student’s t‐test. *P < 0.05, **P < 0.01, ***P < 0.001