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Fig. 4 | Journal of Biomedical Science

Fig. 4

From: Interruption of the long non-coding RNA HOTAIR signaling axis ameliorates chemotherapy-induced cachexia in bladder cancer

Fig. 4

HOTAIR overexpression, cisplatin treatment, and ProT overexpression upregulate pro-inflammatory cytokine expression in human J82 bladder cancer cells. a–e J82 cells transduced with or without lentiviral vectors expressing HOTAIR (J82/HOTAIR-2 and -5) or vector control GFP (J82/GFP) were assessed for HOTAIR expression by RT-qPCR (a), cell proliferation by the MTS assay (b), as well as IL-6 (c), TNF-α (d), and IL-1β (e) transcripts by RT-qPCR. f–h J82 cells were treated with cisplatin (2 μg/ml) for 24 h followed by addition of gefitinib (10 μM), erlotinib (1 μM), or the vehicle (DMSO) for an additional 24 h. Expression of IL-6 (f), TNF-α (g), and IL-1β (h) transcripts were assessed by RT-qPCR. i–k J82 cells transduced with or without lentiviral vectors expressing ProT (J82/ProT) or vector control GFP (J82/GFP) were quantified for IL-6 (i), TNF-α (j), and IL-1β (k) transcripts by RT-qPCR. Values shown are mean ± SD (n = 4–6). The ratios of control cells were arbitrarily set to 100

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