Fig. 1

LSD1 is induced in a cellular model of ALS and plays a role as a transcriptional repressor. A LSD1 immunoreactivity (green) was increased in the nucleus of mSOD1 (G93A) NSC-34 motor neuron-like cells. The nucleus was counterstained with DAPI (blue) and scale bar is 10 μm (white): B Quantification of LSD1 immunoreactivity level. The number of cell counting: 30 cells/group. C H3K4me2 immunoreactivity (green) is decreased in NSC-34/mSOD1 cells. D Quantification of H3K4me2 immunoreactivity level. The number of cell counting: 30 cells/group. E Western blot analysis shows that LSD1 protein level was increased while H3K4me2 protein level was decreased in NSC-34/mSOD1 cells compared to NSC-34 motor neuron cells. F Quantification of LSD1 and H3K4me2 protein level. G LSD1 significantly repressed Gal4-DBD-driven transcriptional activity in SH-SY5Y neuroblastoma cells. Gal4-DBD, Gal4-LSD1, and Gal4-LSD1-DC (a deletion mutant of histone demethylase c-terminus domain) plasmids were transiently transfected together with Gal4-luciferase reporter gene. The data are the means ± SEM of three independent experiments. Significantly different at *p < 0.05 and **p < 0.01