Fig. 3
LSD1 knock-down by shLSD1 increases the level of H3K4me2 but not H3K4me3, and ameliorates neuronal survival. A shLSD1-GFP but not shControl-GFP reduced endogenous LSD1 levels. Arrows indicate GFP-positive cells expressing shRNA. Scale bars (white): 10 μm. The densitometry analysis shows that LSD1 is significantly reduced by shLSD1-GFP (bottom panel). The number of cells counting: 30 cells/group. B shLSD1-GFP decreased H3K4me2 immunoreactivity. The densitometry analysis shows that H3K4me2 is significantly reduced by shLSD1-GFP (bottom panel). The number of cells counting: 20 cells/group. C H3K4me3 immunoreactivity was not altered by shLSD1-GFP expression. Densitometry analysis shows that H3K4me3 was slightly increased by shLSD1-GFP but not significant (bottom panel). The number of cells counting: 20 cells/group. D Knock-down of LSD1 (shLSD1) improved neuronal survival under oxidative stress conditions. shRNA control (shCont) and shLSD1 (500 ng/ml) were transiently transfected in N2a cells for 36 h. Cells were exposed to 100 µM of H2O2 for 12 h and viability was measured by MTT assay. Data was derived from three independent experiments (duplicates in each experiment). Significantly different at *p < 0.05, **p < 0.01, and ***p < 0.001