Fig. 7
mRNAs and associated putative eRNAs expressed at photoreceptor specific gene loci are downregulated in SCA7 homozygous mice. Graphs showing the RT-qPCR analysis of mRNA and associated putative eRNA levels expressed at photoreceptor gene loci in the retina of SCA7140Q/140Q homozygous mice and WT littermates at 12.5 weeks. All mRNAs and annotated putative eRNAs of photoreceptor genes show downregulation in SCA7 retina compared to WT. The non-deregulated Patl1 gene and associated non-deregulated non-coding RNA (based on RNA-seq) were used as control. The non-deregulated Hprt gene which has no associated lncRNA was used as negative, strand specific control. Data are normalized on Rplp0 mRNA expression level, expressed as mean ± SEM (n = 3–6) and analyzed using two-tailed Student’s t-test; *p < 0.05; **p < 0.01; ***p < 0.001