Fig. 2

Histopathology in the dotted ring stage. A Sagittal lens drawing to illustrate the location of the early dot-like microlesions. B H&E stained frontal section of the microlesions in the cortical region, and C at higher magnification. D The location of microlesions in close proximity to LECs and relative to the nuclei of the elongating fibers, and E at higher magnification. F Overview of TUNEL positive nuclei of the elongating fiber cells in frontal sections, and G at higher magnification. H Overview of sagittal section showing apoptotic nuclei of the elongating fiber cells and LECs, and I and J higher magnifications. TUNEL positive cells co-localized with in vivo detectable hyper-reflective microlesions