Fig. 3
From: Pre-hyperglycemia immune cell trafficking underlies subclinical diabetic cataractogenesis
Microstructural lesions in the dotted ring stage and ASC. H&E staining of lenses in the dotted ring stage show A early sub-capsular lesions (black arrows), B amorphous material formation (white arrows) and local LEC proliferation (asterisk), and C vesicular structures inside the lens capsule (black arrowheads). IHC for E-cadherin in lens flatmounts during ASC showed D structural and molecular changes in LECs surrounding ASC lesions (asterisk), E intercellular adhesion defects, F intercellular spaces, and G intracellular vesicle formations. H Cross section of a lens flatmount stained for E-cadherin shows subcapsular amorphous materials. I Intravital microscopy of the anterior part of the lens shows cell contours in areas surrounding the ASC lesion (asterisk), and J extracellular vesicle formations in higher magnifications