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Fig. 4 | Journal of Biomedical Science

Fig. 4

From: The miR-15b-Smurf2-HSP27 axis promotes pulmonary fibrosis

Fig. 4

Inverse correlation between pHSP25 and Smurf2 in BLM-induced fibrosis models. A Analysis of smurf2 mRNA expression level in PBS control mice and lung tissues of BLM-induced lung fibrosis mice analyzed using the GSE18800 (left) and GSE161322 (right) datasets (mean ± SD). **P < 0.01 and ***P < 0.001 vs. PBS control. B Representative images of mouse lung sections stained with H&E or Masson’s trichrome and HSP25 expression. Graphs show scores quantifying inflammation, collagen deposition, and HSP25-positive cells. Magnification, 12.5 × . Scale bar, 100 μm. 200 × . Scale bar, 100 μm. *P < 0.05, **P < 0.01 and ***P < 0.001 vs. PBS control. C Microarray data of smurf2 mRNA (left). Confirmation of smurf2 mRNA by quantitative RT-PCR using lungs from individual mice (right). D Sections from control and BLM-induced lung fibrosis mice tissues were stained for Smurf2 (green), pHSP25 (Red), and α-SMA (violet). Representative images of each specimen are shown. The data from 3 specimens are quantitated. The graph shows the intensity of co-localization of Smurf2 (green) and pHSP25 (red) or pHSP25 (red) and α-SMA (violet) in fibrotic areas from 30 independent views in each sample. Magnification, 400 × . Scale bar, 20 μm. *P < 0.05 and ***P < 0.001 vs. PBS control

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