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Fig. 2 | Journal of Biomedical Science

Fig. 2

From: Inhibition of histone deacetylase 6 destabilizes ERK phosphorylation and suppresses cancer proliferation via modulation of the tubulin acetylation-GRP78 interaction

Fig. 2

Suppression of HDAC6 downregulated ERK phosphorylation. A The protein–protein interaction network of HDAC6 and cancer-related proteins was constructed using the STRING online database. B H460 and C A549 cells were treated with various concentrations of TSA for 4 h. D H460 and E A549 cells were treated with 5 µM TSA for various time points. p-ERK and ERK expressions were examined by Western blot assay. The intensity was normalized to that of GAPDH. *p < 0.05 vs. control cells (n = 3). F H460 and G A549 cells were transfected with siRNA against HDAC6 (siHDAC6) or control siRNA (siCtrl). The protein expressions of HDAC6, p-ERK, and ERK were analyzed by immunoblotting. The intensity was normalized to that of GAPDH. Data are presented as the mean ± SEM. *p < 0.05 vs. siCtrl cells (n = 3)

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Journal of Biomedical Science

ISSN: 1423-0127