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Fig. 3 | Journal of Biomedical Science

Fig. 3

From: Inhibition of histone deacetylase 6 destabilizes ERK phosphorylation and suppresses cancer proliferation via modulation of the tubulin acetylation-GRP78 interaction

Fig. 3

Suppression of HDAC6 upregulated tubulin acetylation. A H460 and B A549 cells were treated with various concentrations of TSA for 4 h. C H460 and D A549 cells were treated with 5 µM TSA for various time points. Acetylated tubulin expression was examined by immunoblotting. The intensity was normalized to that of α-tubulin. *p < 0.05 vs. control cells (n = 3). E H460 and F A549 cells were transfected with siRNA against HDAC6 (siHDAC6) or control siRNA (siCtrl). Acetylated tubulin expression was analyzed by immunoblotting. The intensity was normalized to that of α-tubulin. Data are presented as the mean ± SEM. *p < 0.05 vs. siCtrl cells (n = 3)

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