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Fig. 6 | Journal of Biomedical Science

Fig. 6

From: Inhibition of histone deacetylase 6 destabilizes ERK phosphorylation and suppresses cancer proliferation via modulation of the tubulin acetylation-GRP78 interaction

Fig. 6

Suppression of HDAC6 mediated GRP78-p-ERK detachment from microtubules. A H460 and B A549 cells were treated with 5 µM TSA for 4 h. Lysates were separated into soluble (S) and pellet (P) fractions using a microtubule sedimentation assay and analyzed for GRP78, acetylated tubulin, and α-tubulin by immunoblotting. The ratio of the pellet to the total fraction was calculated. Data are presented as the mean ± SEM. *p < 0.05 vs. control cells (n = 3). C H460 and D A549 cells were transfected with siHDAC6, siGRP78, or control siRNA (siCtrl). The expression levels of GRP78, p-ERK, ERK, and acetylated tubulin were analyzed by immunoblotting. The intensity was normalized to that of GAPDH. Data are presented as the mean ± SEM. *p < 0.05 vs. siCtrl cells; #p < 0.05 vs indicated cells (n = 3)

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Journal of Biomedical Science

ISSN: 1423-0127