Fig. 4
Duration of fecal shedding of P. aeruginosa after prophylactic fucoidans-supplementation. A Timelines of fecal shedding tests were scheduled for 30 days, including 2 days before and 14 days treatment of fucoidans FV (Fucus vesiculosus) and AN (Ascophyllum nodusum) after challenge of Shanghai-fever strain of P. aeruginosa. Negative control (NC) was the mouse challenged with water alone. Oral challenge of P. aeruginosa (1.0 × 107 CFU) was applied for three days from Day-2 to Day 0. Fucoidans FV, and AN (0.5% in water, w/v) were used to feed mice (10 mice per each group) from Day-4 to Day + 14. The horizontal bars indicate median values of CFUs in stool of individual animals at different points in time (days post-infection). (B) Kaplan–Meier curves displaying P. aeruginosa decolonization rates after treatment of fucoidans FV (Fucus vesiculosus) and AN (Ascophyllum nodusum). P. aeruginosa detection in stool was considered positive carrier. P. aeruginosa colonization rates in the gastrointestinal tract at the end of the study in mice were significantly different between the groups with and without fucoidan treatment