Fig. 1

Phenotypes of Rrbp1-KO mice. A Mean value of systolic blood pressure and B diastolic blood pressure in mice. Data represent mean ± SEM, n = 12–49 per group. C Kaplan–Meier and log-rank survival analysis of Rrbp1-WT, Rrbp1-HE and Rrbp1-KO mice. D Representative electrocardiogram (ECG) of Rrbp1-KO and Rrbp1-WT mice. The ECG waveform of #1 and #2 Rrbp1-WT mice and the ECG waveform of terminal rhythms from #3 and #4 Rrbp1-KO mice. E Protocol for assessing the survival rate of mice under high K⁺ intake for 30 days. F Kaplan–Meier survival curve and log-rank survival analysis of Rrbp1-WT and Rrbp1-KO mice under high K⁺ intake for 30 days. G Protocol for telemetry ECG recording. H–P Quantitation of telemetry electrocardiogram (ECG) parameters in Rrbp1-KO and Rrbp1-WTmice with or without high K⁺ intake. RR interval; heart rate; PR interval; P duration; QRS duration; QT interval; correct QT interval; T amplitude; P amplitude (n = 6–7 per group). Representative telemetric ECG waveform of mice two days before and after high K.⁺ intake of Rrbp1-WT and Rrbp1-KO mice (Q). WT, wild-type; KO, knock-out. Data in (A) and (B) were analyzed with ordinary one-way ANOVA; data in (H)–(P) were analyzed with Mann–Whitney test. Data are represented as mean ± SEM. ns, no significance; *P < 0.05