Fig. 5

RRBP1 deficiency reduces renin transport from ER to Golgi apparatus. A–D Representative confocal microscopy images of control and RRBP1 knockdown Calu-6 cells showing renin (green), GOLIM4 (red), and DAPI (blue) after 50 µM of forskolin induction for 0, 60, 120, and 180 min. E Overlap coefficients of renin (green) and GOLIM4 (red) in control and RRBP1 knockdown of Calu-6 cells after 50 µM of forskolin induction for 0, 60, 120, and 180 min (n = 8 per group). Data in (E) were analyzed with an unpaired, two-tailed Student’s t-test. Data are represented as mean ± SEM. *P < 0.05; ***P < 0.001