Fig. 1

Clec5a knockout attenuates ZIKV infectivity in the testicular tissues of mice. Bodyweight (recorded daily) (a) and survival (monitored every 2 days) (b) in male stat1^−/− and stat1^−/−clec5a^−/− mice infected with ZIKV via mosquitoes. c ZIKV titer in serum samples (n ≥ 3) collected at 2 days post-infection (dpi) and in various tissue samples (n ≥ 4) collected at 7 dpi. Plaque assay was used to quantify infectious ZIKV and the limit of detection was estimated by counting numbers of plaque formation in original lysates. No plaque formation was observed in the livers, kidneys and spleens of some ZIKV-infected mice. d Immunohistochemical staining of tissues from the testes and epididymis (caput, corpus, and cauda) collected from ZIKV-infected mice (n ≥ 4) at 7 dpi and from uninfected C57BL/6 mice (n = 3), which were used as controls. Quantitative data are presented as mean ± standard deviation (SD). *p < 0.05. Scale bars: 100 µm