Fig. 4

Loss of CLEC5A attenuates oligospermia following ZIKV infection. Hematoxylin-and-eosin staining histological analysis of tissue sections of the seminiferous tubules (a) in the testis and (b) the mesonephric tubules in the epididymis. c Sperm count for epididymis tissues from mice. d Spermatid density and distribution in the epididymis of ZIKV-infected stat1^−/− mice (n ≥ 4) and stat1^−/−clec5a^−/− mice (n ≥ 5) (7 dpi), with uninfected C57BL/6 mice (n = 6) as negative controls, quantified using Panoramic Viewer software. For a statistical analysis of the distribution of sperm, more than two randomly selected fields of view of the section of the epididymis tissue were assessed for each mouse, and more than three mice were analyzed for each group. Quantitative data are presented as mean ± SD. *p < 0.05; **p < 0.01. Scale bars: 200 μm