Skip to main content
Fig. 3 | Journal of Biomedical Science

Fig. 3

From: Novel, thalidomide-like, non-cereblon binding drug tetrafluorobornylphthalimide mitigates inflammation and brain injury

Fig. 3

TFBP and TFNBP mitigate LPS-induced increases of nitrite and TNF-α in RAW 264.7 cell cultures. Challenge with LPS induced a spike in levels of nitrite and TNF-α, as compared to non-treated control cells (data not shown). Administration of TFBP reduced nitrite expression at the lowest evaluated 100 nM concentration (B), whereas elevated levels of TNF-α were mitigated starting at 600 nM (C). TFBP was well tolerated and was without impact on cell viability (A). Treatment with TFNBP was likewise effective in decreasing levels of nitrite and TNF-α, without affecting cell viability (Fig. 2D–F). *p < 0.05; **p < 0.01; ****p < 0.0001 vs. cnt-dmso group. N = 4 per group

Back to article page