Fig. 4

Phylogenetic analyses of the Enterovirus. Phylogenetic analyses of the EV-A71 (A), CVA16 (B), CVA6 (C) and CVA10 (D) circulating globally based on full length sequence of the VP1 gene worldwide available from GenBank were conducted in MEGA 7 using the neighbor-joining method. The bootstrap test was performed with 1000 replications. The evolutionary distances were written on the branch. We selected the representative VP1 sequences (EV-A71, n = 84; CVA16, n = 47; CVA6, n = 39; and CVA10, n = 56) from GenBank according to the country of origin, year of isolation and other information. All the strains are labelled using the following format: ‘accession number’/ ‘country of origin’/ ‘year of isolation’. All selected representative strains are marked with distinct colors according to different genotypes/sub-genotypes. The prototypes strains marked with yellow circles and red circles indicate the outgroups. The genotyping reference strains of different genotypes/sub-genotypes of CVA6 and CVA10 are marked as black triangles