Fig. 5

PES1 interacts with ILF3. A Immunoprecipitation with anti-Flag M2 beads in EC9706 cells followed by coomassie brilliant blue staining assay, arrow indicates PES1 and ILF3. B, C Co-immunoprecipitation assay in 293 T cells co-transfected with Flag-PES1 and HA-ILF3. D, E Examination of the interaction between endogenous PES1 and ILF3 by co-immunoprecipitation in EC9706 cells. F Representative PLA images detecting the interaction between endogenous PES1 and ILF3 in normal and tumour tissues. Scale Bar = 100 μm (upper) and 50 μm (lower). G Co-IP was used to identify the ILF3 binding site in PES1. The full-length PES1 and deletion mutant constructs were shown. PES1, ILF3 and their corresponding deletion mutants were co-transfected into 293 T cells. 48 h later, the cells were collected for Co-IP. H, I 293 T cells were transfected with control vector and PES1, PES1 mut, HA-ILF3 plasmid, respectively. After 48 h, co-immunoprecipitation assay was performed. J, K The expression of IL15 in EC9706 cells was examined using qPCR and western blots. **p < 0.01